This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy. The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers. By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.
University of Florida. Fluorescence Correlation Spectroscopy. University of Florida, March 27, 2016. https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13965&DocID=4356 (accessed 20 May 2022).
%0 Electronic Source %D March 27, 2016 %T Fluorescence Correlation Spectroscopy %I University of Florida %V 2022 %N 20 May 2022 %8 March 27, 2016 %9 application/pdf %U https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13965&DocID=4356
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