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Fluorescence Correlation Spectroscopy
published by the University of Florida
This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy.  The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers.  By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.
Subjects Levels Resource Types
Modern Physics
- Biophysics
- Modern Optics
= Lasers
Thermo & Stat Mech
- Kinetic and Diffusive Processes
= Diffusion
- Upper Undergraduate
- Lower Undergraduate
- Instructional Material
= Instructor Guide/Manual
= Laboratory
= Student Guide
Material Category Formats Ratings
- Lab Manual
- application/pdf
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Access Rights:
Free access
© 2015 The University of Florida
Record Creator:
Metadata instance created January 6, 2016 by Stephen Hagen
Record Updated:
March 27, 2016 by Ramon Torres-Isea
Last Update
when Cataloged:
March 27, 2016
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AIP Format
(University of Florida, 2015), WWW Document, (
Fluorescence Correlation Spectroscopy (University of Florida, 2015), <>.
APA Format
Fluorescence Correlation Spectroscopy. (2016, March 27). Retrieved June 21, 2024, from University of Florida:
Chicago Format
University of Florida. Fluorescence Correlation Spectroscopy. University of Florida, March 27, 2016. (accessed 21 June 2024).
MLA Format
Fluorescence Correlation Spectroscopy. University of Florida, 2015. 27 Mar. 2016. 21 June 2024 <>.
BibTeX Export Format
@misc{ Title = {Fluorescence Correlation Spectroscopy}, Publisher = {University of Florida}, Volume = {2024}, Number = {21 June 2024}, Month = {March 27, 2016}, Year = {2015} }
Refer Export Format

%T Fluorescence Correlation Spectroscopy %D March 27, 2016 %I University of Florida %U %O application/pdf

EndNote Export Format

%0 Electronic Source %D March 27, 2016 %T Fluorescence Correlation Spectroscopy %I University of Florida %V 2024 %N 21 June 2024 %8 March 27, 2016 %9 application/pdf %U

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