This manual describes an advanced undergraduate laboratory experiment in fluorescence correlation spectroscopy. The experiment uses a single green laser with avalanche photodiode detectors and a software autocorrelator to study the diffusion of fluorescent spheres as well as fluorescently-labeled DNA oligomers. By analysis of the autocorrelation function students can determine the diffusion coefficient of the particles and observe dynamics in the DNA oligomer.
<a href="https://advlabs.aapt.org/items/detail.cfm?ID=13965">University of Florida. Fluorescence Correlation Spectroscopy. University of Florida, March 27, 2016.</a>
Fluorescence Correlation Spectroscopy. (2016, March 27). Retrieved December 13, 2024, from University of Florida: https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13965&DocID=4356
University of Florida. Fluorescence Correlation Spectroscopy. University of Florida, March 27, 2016. https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13965&DocID=4356 (accessed 13 December 2024).
Fluorescence Correlation Spectroscopy. University of Florida, 2015. 27 Mar. 2016. 13 Dec. 2024 <https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13965&DocID=4356>.
%0 Electronic Source %D March 27, 2016 %T Fluorescence Correlation Spectroscopy %I University of Florida %V 2024 %N 13 December 2024 %8 March 27, 2016 %9 application/pdf %U https://www.compadre.org/Repository/document/ServeFile.cfm?ID=13965&DocID=4356
Disclaimer: ComPADRE offers citation styles as a guide only. We cannot offer interpretations about citations as this is an automated procedure. Please refer to the style manuals in the Citation Source Information area for clarifications.
Record Link
Save to my folders