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Optical Tweezer
published by the University of Florida
This manual describes an advanced undergraduate laboratory experiment in the use of optical tweezers.  The experiment uses a commercially available optical tweezer apparatus based on an infrared diode laser with visible light illumination and quadrant photodetection.  Using this system the student can characterize the strength of the optical trap, observe Brownian motion of a particle in the trap, and explore the biological forces associated with flagellar swimming of bacteria and vesicle transport in a plant cell.  The physics behind the experiment, including the power spectrum of the particle's motion in the trap and the method of trap calibration, are described in detail.
Subjects Levels Resource Types
Modern Physics
- Biophysics
- Color
= Dispersion
= Scattering
- Modern Optics
= Lasers
- Photometry
= Radiation Pressure
Quantum Physics
- Quantum Experiments
Thermo & Stat Mech
- Kinetic and Diffusive Processes
= Brownian Motion
- Upper Undergraduate
- Lower Undergraduate
- Instructional Material
= Instructor Guide/Manual
= Laboratory
= Student Guide
Material Category Formats Ratings
- Lab Manual
- application/pdf
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Safety Warnings
Advanced Labs Terms of Use Disclaimer Eye Protection Must be Worn   Laser Beam  

Access Rights:
Free access
© 2015 University of Florida
Record Creator:
Metadata instance created December 28, 2015 by Stephen Hagen
Record Updated:
March 26, 2016 by Ramon Torres-Isea
Last Update
when Cataloged:
December 31, 2015
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AIP Format
(University of Florida, 2015), WWW Document, (
Optical Tweezer (University of Florida, 2015), <>.
APA Format
Optical Tweezer. (2015, December 31). Retrieved June 15, 2024, from University of Florida:
Chicago Format
University of Florida. Optical Tweezer. University of Florida, December 31, 2015. (accessed 15 June 2024).
MLA Format
Optical Tweezer. University of Florida, 2015. 31 Dec. 2015. 15 June 2024 <>.
BibTeX Export Format
@misc{ Title = {Optical Tweezer}, Publisher = {University of Florida}, Volume = {2024}, Number = {15 June 2024}, Month = {December 31, 2015}, Year = {2015} }
Refer Export Format

%T Optical Tweezer %D December 31, 2015 %I University of Florida %U %O application/pdf

EndNote Export Format

%0 Electronic Source %D December 31, 2015 %T Optical Tweezer %I University of Florida %V 2024 %N 15 June 2024 %8 December 31, 2015 %9 application/pdf %U

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